3D Printing of Monolithic Proteinaceous Cantilevers Using Regenerated Silk Fibroin

Abstract

Silk fibroin, regenerated from Bombyx mori, has shown considerable promise as a printable, aqueous-based ink using a bioinspired salt-bath system in our previous work. Here, we further developed and characterized silk fibroin inks that exhibit concentration-dependent fluorescence spectra at the molecular level. These insights supported extrusion-based 3D printing using concentrated silk fibroin solutions as printing inks. 3D monolithic proteinaceous structures with high aspect ratios were successfully printed using these approaches, including cantilevers only supported at one end. This work provides further insight and broadens the utility of 3D printing with silk fibroin inks for the microfabrication of proteinaceous structures.

Document Details

Document Type
Pub Defense Publication
Publication Date
Mar 26, 2022
Source ID
10.3390/molecules27072148

Entities

People

  • Constancio Gonzalez-obeso
  • David L. Kaplan
  • Gang Li
  • Jugal Kishore Sahoo
  • Peggy Cebe
  • Xuan Mu
  • Yu Shrike Zhang
  • Zhiyu Xia

Organizations

  • Air Force Office of Scientific Research
  • National Institutes of Health
  • National Science Foundation

Tags

Readers

  • Manufacturing Engineering.
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Biotechnology