Targeting Collagen Type III in Proteinuric Kidney Disease: Informing Drug Potential Using the Jaccard–Tanimoto Index
Abstract
Collagenofibrotic glomerulopathy, a collagen type III kidney disease, is associated with proteinuria and accumulation ofcollagen type III in the glomerulus specifically the mesangium and/or capillary walls. The puromcyin aminonucleoside (PAN) nephropathy model was evaluated to examine the relation between COL3A1 mRNA and proteinuria. In Wistar rats administered PAN, a robust increase in proteinuria was accompanied by glomerular hypertrophy and expansion of both the Bowman’s capsule and Bowman’s space. An ~4-fold increase in renal COL3A1 mRNA was observed in the PAN cohort with urine protein exhibiting a direct (r = 0.8) and significant correlation with kidney COL3A1 mRNA level. Both Picrosirius red polarized microscopy and immunohistochemical analysis showed localization of collagen type III to the glomerular mesangium. Gene ontology-driven transcriptomic analysis reveals a robust COL3A1 network in the glomerular compartment.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 16, 2020
- Source ID
- 10.3390/pr8080996
Entities
People
- Anoushka Dalvi
- Anthony Pellicano
- Itzhak D. Goldberg
- Kai Jiang
- Michelle Liu
- Natalia Prakash
- Ping Zhou
- Prakash Narayan
- Sony Dalapati
- Zhijian Hu
Organizations
- United States Department of Defense