Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats (Eonycteris spelaea) in Singapore
Abstract
Rousettus bat coronavirus GCCDC1 (RoBat-CoV GCCDC1) is a cross-family recombinant coronavirus that has previously only been reported in wild-caught bats in Yúnnan, China. We report the persistence of a related strain in a captive colony of lesser dawn bats captured in Singapore. Genomic evidence of the virus was detected using targeted enrichment sequencing, and further investigated using deeper, unbiased high throughput sequencing. RoBat-CoV GCCDC1 Singapore shared 96.52% similarity with RoBat-CoV GCCDC1 356 (NC_030886) at the nucleotide level, and had a high prevalence in the captive bat colony. It was detected at five out of six sampling time points across the course of 18 months. A partial segment 1 from an ancestral Pteropine orthoreovirus, p10, makes up the recombinant portion of the virus, which shares high similarity with previously reported RoBat-CoV GCCDC1 strains that were detected in Yúnnan, China. RoBat-CoV GCCDC1 is an intriguing, cross-family recombinant virus, with a geographical range that expands farther than was previously known. The discovery of RoBat-CoV GCCDC1 in Singapore indicates that this recombinant coronavirus exists in a broad geographical range, and can persist in bat colonies long-term.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 14, 2020
- Source ID
- 10.3390/v12050539
Entities
People
- Adrian C. Paskey
- Casandra W. Philipson
- Danielle E Anderson
- Eric D. Laing
- Gavin J.D. Smith
- Gregory K. Rice
- Ian H Mendenhall
- Justin H. J. Ng
- Kenneth G. Frey
- Kimberly A Bishop-Lilly
- Kyle A. Long
- Lin-Fa Wang
- Matthew R. Lueder
- Randy J.h. Foo
- Regina Z Cer
- Theron Hamilton
- Wan Ni Chia
- Xiao Fang Lim
Organizations
- United States Navy