Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles

Abstract

Clathrin-mediated endocytosis (CME) plays a fundamental role in podocyte health. Genetic ablation of genes implicated in CME has been shown to cause severe proteinuria and foot process effacement in mice. However, little is known about the cargo of clathrin-coated vesicles (CCVs) in podocytes. The goal of this study was to isolate CCVs from podocytes and identify their cargo by proteomic analysis.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jun 01, 2020
Source ID
10.34067/kid.0000212020

Entities

People

  • Christopher E. Pedigo
  • Elizabeth Cross
  • Eunice Baik
  • Karen Ebenezer
  • Kazunori Inoue
  • Mario Schiffer
  • Marwin Groener
  • Shuta Ishibe
  • Xuefei Tian
  • Ying Wang

Organizations

  • Friedrich-Alexander-Universität Erlangen-Nürnberg
  • German Academic Exchange Service
  • National Institutes of Health
  • United States Department of Defense
  • University Hospital Erlangen
  • Yale School of Medicine
  • Yale University

Tags

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular and Cellular Biochemistry
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech