Cutting Edge: Distinct B Cell Repertoires Characterize Patients with Mild and Severe COVID-19

Abstract

Protective immunity against COVID-19 likely depends on the production of SARS-CoV-2–specific plasma cells and memory B cells postinfection or postvaccination. Previous work has found that germinal center reactions are disrupted in severe COVID-19. This may adversely affect long-term immunity against reinfection. Consistent with an extrafollicular B cell response, patients with severe COVID-19 have elevated frequencies of clonally expanded, class-switched, unmutated plasmablasts. However, it is unclear whether B cell populations in individuals with mild COVID-19 are similarly skewed. In this study, we use single-cell RNA sequencing of B cells to show that in contrast to patients with severe COVID-19, subjects with mildly symptomatic COVID-19 have B cell repertoires enriched for clonally diverse, somatically hypermutated memory B cells ∼30 d after the onset of symptoms. This provides evidence that B cell responses are less disrupted in mild COVID-19 and result in the production of memory B cells.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jun 15, 2021
Source ID
10.4049/jimmunol.2100135

Entities

People

  • Alexander Bukreyev
  • Avraham Unterman
  • Charles S Dela Cruz
  • David A. Hafler
  • Hiromitsu Asashima
  • Kenneth B. Hoehn
  • Naftali Kaminski
  • Palani Ramanathan
  • Steven H Kleinstein
  • Stuart C. Sealfon
  • Tomokazu Sumida

Organizations

  • Icahn School of Medicine at Mount Sinai
  • National Heart, Lung, and Blood Institute
  • National Institute of Allergy and Infectious Diseases
  • University of Texas Medical Branch
  • Yale School of Medicine
  • Yale University

Tags

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Molecular and genetic basis of cancer.
  • Virology (or Medical Virology).