From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols
Abstract
The clinical application of human-induced pluripotent stem cells (hiPSCs) requires not only the production of Good Manufacturing Practice-grade (GMP-grade) hiPSCs but also the derivation of specified cell types for transplantation under GMP conditions. Previous reports have suggested that hiPSCs can be produced in the absence of animal-derived reagents (xenobiotics) to ease the transition to production under GMP standards. However, to facilitate the use of hiPSCs in cell-based therapeutics, their progeny should be produced not only in the absence of xenobiotics but also under GMP conditions requiring extensive standardization of protocols, documentation, and reproducibility of methods and product. Here, we present a successful framework to produce GMP-grade derivatives of hiPSCs that are free of xenobiotic exposure from the collection of patient fibroblasts, through reprogramming, maintenance of hiPSCs, identification of reprogramming vector integration sites (nrLAM-PCR), and finally specification and terminal differentiation of clinically relevant cells. Furthermore, we developed a primary set of Standard Operating Procedures for the GMP-grade derivation and differentiation of these cells as a resource to facilitate widespread adoption of these practices.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 07, 2011
- Source ID
- 10.5966/sctm.2011-0001
Entities
People
- Aaron R. Cooper
- Amander Clark
- April Pyle
- Donald B. Kohn
- James Byrne
- Jerome A. Zack
- Kathrin Plath
- Michaela Patterson
- Peiyee Lee
- Saravanan Karumbayaram
- Soheila F. Azghadi
- William E. Lowry
Organizations
- National Institutes of Health
- United States Department of Defense
- University of California, Los Angeles