The genome sequence of the colonial chordate, Botryllus schlosseri
Abstract
Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 02, 2013
- Source ID
- 10.7554/elife.00569
Entities
People
- Aaron M. Newman
- Ayelet Voskoboynik
- Benedetto Passarelli
- Carmela Gissi
- Daniel M Corey
- Debashis Sahoo
- Dmitry Pushkarev
- Francesca Griggio
- Gary L Mantalas
- H Christina Fan
- Irving L Weissman
- Karla J Palmeri
- Katherine J Ishizuka
- Lolita Penland
- Norma F Neff
- Rachel Ben-shlomo
- Richard A White Iii
- Stephen Quake
- Winston Koh
Organizations
- Ludwig Institute for Cancer Research
- National Institutes of Health
- Stanford University
- Thomas and Stacey Siebel Foundation
- United States Department of Defense
- University of Milan