A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis

Abstract

mir-17-92, a potent polycistronic oncomir, encodes six mature miRNAs with complex modes of interactions. In the Eμ-myc Burkitt’s lymphoma model, mir-17-92 exhibits potent oncogenic activity by repressing c-Myc-induced apoptosis, primarily through its miR-19 components. Surprisingly, mir-17-92 also encodes the miR-92 component that negatively regulates its oncogenic cooperation with c-Myc. This miR-92 effect is, at least in part, mediated by its direct repression of Fbw7, which promotes the proteosomal degradation of c-Myc. Thus, overexpressing miR-92 leads to aberrant c-Myc increase, imposing a strong coupling between excessive proliferation and p53-dependent apoptosis. Interestingly, miR-92 antagonizes the oncogenic miR-19 miRNAs; and such functional interaction coordinates proliferation and apoptosis during c-Myc-induced oncogenesis. This miR-19:miR-92 antagonism is disrupted in B-lymphoma cells that favor a greater increase of miR-19 over miR-92. Altogether, we suggest a new paradigm whereby the unique gene structure of a polycistronic oncomir confers an intricate balance between oncogene and tumor suppressor crosstalk.

Document Details

Document Type
Pub Defense Publication
Publication Date
Oct 15, 2013
Source ID
10.7554/elife.00822

Entities

People

  • Alex C Minella
  • Alicia Y Zhou
  • Andrei Goga
  • Anne Biton
  • Asha Balakrishnan
  • Caitlin S De Jong
  • Erich Sabio
  • Gerard I. Evan
  • James C Mcgann
  • Lin He
  • Margaux J Bennett
  • Micah A Luftig
  • Mona Foth
  • Nicole M Sodir
  • Samantha K Greaney
  • Terence P. Speed
  • Virginie Olive
  • Ying Wan
  • Zhenyu Xuan

Organizations

  • American Cancer Society
  • Army Medical University
  • Duke University
  • Istituto Superiore di Sanità
  • Leukemia & Lymphoma Society
  • National Cancer Institute
  • National Heart, Lung, and Blood Institute
  • National Institutes of Health
  • Northwestern University
  • Statistics New Zealand
  • Tobacco-Related Disease Research Program
  • United States Department of Defense
  • University of California
  • University of Cambridge
  • University of Texas at Dallas

Tags

Readers

  • Breast cancer cell signaling and growth regulation.
  • Nuclear Non-Proliferation and International Security
  • Oncology (Cancer Research).