Mycobacterium tuberculosis induces decelerated bioenergetic metabolism in human macrophages
Abstract
How Mycobacterium tuberculosis (Mtb) rewires macrophage energy metabolism to facilitate survival is poorly characterized. Here, we used extracellular flux analysis to simultaneously measure the rates of glycolysis and respiration in real time. Mtb infection induced a quiescent energy phenotype in human monocyte-derived macrophages and decelerated flux through glycolysis and the TCA cycle. In contrast, infection with the vaccine strain, M. bovis BCG, or dead Mtb induced glycolytic phenotypes with greater flux. Furthermore, Mtb reduced the mitochondrial dependency on glucose and increased the mitochondrial dependency on fatty acids, shifting this dependency from endogenous fatty acids in uninfected cells to exogenous fatty acids in infected macrophages. We demonstrate how quantifiable bioenergetic parameters of the host can be used to accurately measure and track disease, which will enable rapid quantifiable assessment of drug and vaccine efficacy. Our findings uncover new paradigms for understanding the bioenergetic basis of host metabolic reprogramming by Mtb.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 16, 2018
- Source ID
- 10.7554/elife.39169
Entities
People
- Adrie J C Steyn
- Bridgette M Cumming
- John H. Adamson
- Kelvin W Addicott
Organizations
- Gates Foundation
- National Institutes of Health
- South African Medical Research Council
- United States Department of Defense
- University of Alabama at Birmingham
- University of Alabama at Birmingham School of Medicine