Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway
Abstract
High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 19, 2018
- Source ID
- 10.7554/elife.40444
Entities
People
- Anh M Trinh
- John C Chaput
- Lynnette N Jackson
- Nicholas Chim
Organizations
- Defense Advanced Research Projects Agency
- National Institutes of Health
- National Science Foundation
- University of California