Quantitative regulation of the dynamic steady state of actin networks
Abstract
Principles of regulation of actin network dimensions are fundamentally important for cell functions, yet remain unclear. Using both in vitro and in silico approaches, we studied the effect of key parameters, such as actin density, ADF/Cofilin concentration and network width on the network length. In the presence of ADF/Cofilin, networks reached equilibrium and became treadmilling. At the trailing edge, the network disintegrated into large fragments. A mathematical model predicts the network length as a function of width, actin and ADF/Cofilin concentrations. Local depletion of ADF/Cofilin by binding to actin is significant, leading to wider networks growing longer. A single rate of breaking network nodes, proportional to ADF/Cofilin density and inversely proportional to the square of the actin density, can account for the disassembly dynamics. Selective disassembly of heterogeneous networks by ADF/Cofilin controls steering during motility. Our results establish general principles on how the dynamic steady state of actin network emerges from biochemical and structural feedbacks.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 14, 2019
- Source ID
- 10.7554/elife.42413
Entities
People
- Alex Mogilner
- Angelika Manhart
- Christophe Guerin
- Laurent Blanchoin
- Manuel Théry
- Rajaa Boujemaa-paterski
- Tobbias Klar
- Téa Aleksandra Icheva
Organizations
- Agence Nationale de la Recherche
- Army Research Office
- Courant Institute of Mathematical Sciences, NYU
- Grenoble Alpes University
- New York University