Flower-like patterns in multi-species bacterial colonies

Abstract

Diverse interactions among species within bacterial colonies lead to intricate spatiotemporal dynamics, which can affect their growth and survival. Here, we describe the emergence of complex structures in a colony grown from mixtures of motile and non-motile bacterial species on a soft agar surface. Time-lapse imaging shows that non-motile bacteria 'hitchhike' on the motile bacteria as the latter migrate outward. The non-motile bacteria accumulate at the boundary of the colony and trigger an instability that leaves behind striking flower-like patterns. The mechanism of the front instability governing this pattern formation is elucidated by a mathematical model for the frictional motion of the colony interface, with friction depending on the local concentration of the non-motile species. A more elaborate two-dimensional phase-field model that explicitly accounts for the interplay between growth, mechanical stress from the motile species, and friction provided by the non-motile species, fully reproduces the observed flower-like patterns.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 14, 2020
Source ID
10.7554/elife.48885

Entities

People

  • Jeff Hasty
  • Lev S Tsimring
  • Liyang Xiong
  • Robert M Cooper
  • Wouter-Jan Rappel
  • Yuansheng Cao

Organizations

  • National Institutes of Health
  • National Science Foundation
  • Office of Naval Research
  • University of California
  • University of California, San Diego

Tags

Fields of Study

  • Biology
  • Environmental science
  • Mathematics

Readers

  • Immunology
  • Molecular and Cellular Biology
  • Nanocomposite Materials Science