A bacterial membrane sculpting protein with BAR domain-like activity
Abstract
Bin/Amphiphysin/RVS (BAR) domain proteins belong to a superfamily of coiled-coil proteins influencing membrane curvature in eukaryotes and are associated with vesicle biogenesis, vesicle-mediated protein trafficking, and intracellular signaling. Here, we report a bacterial protein with BAR domain-like activity, BdpA, from Shewanella oneidensis MR-1, known to produce redox-active membrane vesicles and micrometer-scale outer membrane extensions (OMEs). BdpA is required for uniform size distribution of membrane vesicles and influences scaffolding of OMEs into a consistent diameter and curvature. Cryo-TEM reveals that a strain lacking BdpA produces lobed, disordered OMEs rather than membrane tubules or narrow chains produced by the wild-type strain. Overexpression of BdpA promotes OME formation during planktonic growth of S. oneidensis where they are not typically observed. Heterologous expression results in OME production in Marinobacter atlanticus and Escherichia coli. Based on the ability of BdpA to alter membrane architecture in vivo, we propose that BdpA and its homologs comprise a newly identified class of bacterial BAR domain-like proteins.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 13, 2021
- Source ID
- 10.7554/elife.60049
Entities
People
- Aleksandr E. Miklos
- Anthony P. Malanoski
- Brian J Eddie
- Cheri M Hampton
- Daniel A Phillips
- Grace W Chong
- Grant Jensen
- Lauren Ann Metskas
- Lawrence F Drummy
- Lina J. Bird
- Lori Zacharoff
- Mohamed Y El-Naggar
- Sarah M Strycharz
- Shuai Xu
Organizations
- Air Force Research Laboratory
- Brigham Young University
- California Institute of Technology
- National Institute of General Medical Sciences
- National Science Foundation
- Oak Ridge Institute for Science and Education
- Office of Naval Research
- United States Army Combat Capabilities Development Command
- United States Department of Defense
- United States Department of Energy
- United States Naval Research Laboratory
- University of Southern California
- University of the Philippines Los Baños College of Development Communication