Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations
Abstract
Laboratory experimental evolution provides a window into the details of the evolutionary process. To investigate the consequences of long-term adaptation, we evolved 205 Saccharomyces cerevisiae populations (124 haploid and 81 diploid) for ~10,000 generations in three environments. We measured the dynamics of fitness changes over time, finding repeatable patterns of declining adaptability. Sequencing revealed that this phenotypic adaptation is coupled with a steady accumulation of mutations, widespread genetic parallelism, and historical contingency. In contrast to long-term evolution in E. coli, we do not observe long-term coexistence or populations with highly elevated mutation rates. We find that evolution in diploid populations involves both fixation of heterozygous mutations and frequent loss-of-heterozygosity events. Together, these results help distinguish aspects of evolutionary dynamics that are likely to be general features of adaptation across many systems from those that are specific to individual organisms and environmental conditions.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 19, 2021
- Source ID
- 10.7554/elife.63910
Entities
People
- Alex N Nguyen Ba
- Alief Moulana
- Angela M Phillips
- Artur Rego-costa
- Christopher W. Bakerlee
- Elizabeth R Jerison
- Jiseon Min
- Juhee Goyal
- Julia C Piper
- Katherine R. Lawrence
- Katya Kosheleva
- Megan E Dillingham
- Michael J. Mcdonald
- Michael M Desai
- Milo S Johnson
- Parris T Humphrey
- Ramya Purkanti
- Shreyas Gopalakrishnan
- Tanush Jagdish
Organizations
- Australian Research Council
- Harvard University
- Hertz Foundation
- Massachusetts Institute of Technology
- Max Planck Institute of Molecular Cell Biology and Genetics
- Ministry of Science and Technology
- Monash University
- National Institutes of Health
- National Science Foundation
- Natural Sciences and Engineering Research Council
- Simons Foundation
- Stanford University
- University of Toronto