Bulk flow of cerebrospinal fluid observed in periarterial spaces is not an artifact of injection
Abstract
Cerebrospinal fluid (CSF) flowing through periarterial spaces is integral to the brain’s mechanism for clearing metabolic waste products. Experiments that track tracer particles injected into the cisterna magna (CM) of mouse brains have shown evidence of pulsatile CSF flow in perivascular spaces surrounding pial arteries, with a bulk flow in the same direction as blood flow. However, the driving mechanism remains elusive. Several studies have suggested that the bulk flow might be an artifact, driven by the injection itself. Here, we address this hypothesis with new in vivo experiments where tracer particles are injected into the CM using a dual-syringe system, with simultaneous injection and withdrawal of equal amounts of fluid. This method produces no net increase in CSF volume and no significant increase in intracranial pressure. Yet, particle-tracking reveals flows that are consistent in all respects with the flows observed in earlier experiments with single-syringe injection.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 09, 2021
- Source ID
- 10.7554/elife.65958
Entities
People
- Aditya Raghunandan
- Antonio Ladrón-de-Guevara
- Douglas H Kelley
- Humberto Mestre
- Jeffrey Tithof
- John H Thomas
- Maiken Nedergaard
- Ting Du
Organizations
- Army Research Office
- Burroughs Wellcome Fund
- National Institutes of Health
- University of Copenhagen
- University of Minnesota
- University of Rochester