Three-dimensional multi-site random access photostimulation (3D-MAP)
Abstract
Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 14, 2022
- Source ID
- 10.7554/elife.73266
Entities
People
- Hillel Adesnik
- Laura Waller
- Nicolas C Pégard
- Yi Xue
Organizations
- Arnold and Mabel Beckman Foundation
- Burroughs Wellcome Fund
- Defense Advanced Research Projects Agency
- Gordon and Betty Moore Foundation
- National Institutes of Health
- New York Stem Cell Foundation
- University of California, Berkeley
- University of North Carolina at Chapel Hill