Biodosimetry (USUHS)
Abstract
Biodosimetry (USUHS/AFRRI): Research findings are focused to advance the development and to produce the following: (1) To establish clinically certified processes to permit rapid assessment of radiation exposed specimens; (2) To access radiation exposure by developing and providing biological and biophysical dosimetry capabilities for acute, protracted, and prior radiation exposure; (3) To develop novel triage protocols for rapid assessment of radiation exposure; (4) To establish equipment triage automation to support the ability to manage mass-casualty radiation incidents around the globe. In addition to the primary achievement of research objectives, the program educates Federal employees as a benefit to the public they serve through Federal service, through support to civil authorities, and in non-Federal professional and academic collaborations. Description: Biodosimetry (USUHS/AFRRI): Research findings are focused to advance the development and to produce the following: (1) To establish clinically certified processes to permit rapid assessment of radiation exposed specimens; (2) To access radiation exposure by developing and providing biological and biophysical dosimetry capabilities for acute, protracted, and prior radiation exposure; (3) To develop novel triage protocols for rapid assessment of radiation exposure; (4) To establish equipment triage automation to support the ability to manage mass-casualty radiation incidents around the globe. In addition to the primary achievement of research objectives, the program educates Federal employees as a benefit to the public they serve through Federal service, through support to civil authorities, and in non-Federal professional and academic collaborations. FY21 Accomplishments: (1)Validated the HIRI algorithm concept using animals (i.e., baboons, canine, and mice) dose-response databases for an extended time window up to 10 to 14 days after exposure. (2)Established a RICA algorithm for assessment of H-ARS severity using NHP radiation dose-response model based on CBC cell types biomarkers using the METREPOL system. (3)Developed a quantitative inhibition PCR assay of nuclear and mitochondrial DNA using long- and short amplicon PCR. (4)Evaluated radiation-dose and time-course response following exposure initially to photon irradiation. (5)Compared gamma ray v/s neutron mixed field exposures on the inhibition PCR assay. (6)Characterized the utility of hematology biodosimetry algorithms (i.e., HIRI, RICA) to access radiation injury after radiation exposures. (7)Established a quantitative inhibition PCR of DNA damage using blood lymphocyte models and characterize its utility for assessment of radiation exposure. (8)Performed simulated in vitro partial-body exposure studies and use cytogenetic biomarkers (PCC assay). (9)Evaluated the PCC endpoints (i.e., excess PCC fragments, lengths ratios, rings, and dicentric) for optimum assessment of the fraction of body exposed and dose to the irradiated fraction using in vivo human blood lymphocytes model. (10)Investigated gamma rays v/s mixed field exposures on PCC assay to distinguish whole-body v/s partial body high dose radiation exposures. (11)In 2019/2021, 15 manuscripts were published.
Document Details
- Document Type
- Accomplishment
- Publication Date
- Oct 01, 2023
- Source ID
- 8ff9ee5ef88610f5d3ad682c36602fe6