INVESTIGATIONS OF THE PATHWAY OF ACETATE AND SUCCINATE OXIDATION BY ESCHERICHIA COLI
Abstract
The oxidation of succinate by E. coli, strain B, was completely suppressed at concentrations (4 x 10 to the -4th power M) of 2,4-dinitrophenol (DNP) which permitted ready oxidation of the acetate. The oxidative assimilation of acetate was totally inhibited at DNP contents which permitted succinate oxidation. Acetate-grown cells oxidized acetate at a greater rate than succinate- or nutrient broth-grown cells. However, the rates of succinate oxidation were about the same with cells grown either on acetate, succinate, or nutrient broth. The results indicated that E. coli did not form succinate as an intermediate in the oxidation of acetate of CO sub2 and H sub2 O, and that neither a di-nor a tricarboxylic acid cycle appeared to be a major pathway for acetate oxidation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 25, 1953
- Accession Number
- AD0012493
Entities
People
- Charles R. Goucher
- Eugene E. Woodside
- Walter F. Kocholaty
Organizations
- United States Army Medical Research Laboratory