STUDY OF CHEMICAL METHODS FOR QUANTITATIVE MEASUREMENTS OF CATECHOL AMINES

Abstract

A method for the estimation of adrenaline and noradrenaline in urine is presented. This method employs an ion exchange resin, Amberlite XE-64, for purification. The simultaneous estimation of the two hormones is accomplished by a multiple filter technique of fluorometry. The selection of appropriate sets of filters is based on a careful study of the fluorescence spectra of the compound concerned. The method has been subjected to careful scrutiny as to factors affecting reproducibility and precision of the measurements. Evidence is presented that a high degree of specificity has been attained.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1959
Accession Number
AD0220081

Entities

People

  • Charles H. Dutoit

Organizations

  • Massachusetts General Hospital

Tags

DTIC Thesaurus Topics

  • Acids
  • Air Force
  • Amines
  • Amino Acids
  • Bioassay
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Emission Spectra
  • Fluorescence
  • Ion Exchange
  • Materials
  • Measurement
  • Medical Laboratories
  • Spectra
  • Standards
  • Vitamin C

Readers

  • Adaptive Control and Estimation with Uncertainty in Dynamic Systems.
  • Analytical Chemistry
  • Cardiovascular Physiology