STABILITY OF LIQUID CULTURES OF COCCIDIOIDES IMMITIS ARTHROSPORES TO STORAGE, DRYING, AND FREEZING AND THAWING

Abstract

Optimum storage stability at 5 C was obtained with 14-day old arthrospores supplemented with glucose and ammonium acetate or harvested from synthetic medium and resuspended in 8 per cent glucose. The ratio of the air space to culture volume within the storage container was critical, with best stability obtained at a ratio of 1, or less than 1. Disturbance of the storage containers (i.e., shaking or opening) during storage had no effect on viability. Resuspension of arthrospores in 8% glucose markedly increased survival to drying, freezing and thawing, and aerosolization. During the freezing and thawing cycle, the temperature of freezing was more critical for survival than the temperature of thawing.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1959
Accession Number
AD0252751

Entities

People

  • Arthur R. Besener
  • Ernest M. Snyder
  • Henry F. Tantalo
  • John L. Converse

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Air Platforms
  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Aerosols
  • Age Groups
  • Base Lines
  • Biological Laboratories
  • Calcium Compounds
  • Cells
  • Containers
  • Freezing
  • Fungi
  • Glycerols
  • Intact Stability
  • Intervals
  • Liquids
  • Low Temperature
  • Sodium
  • Standards
  • Sugar Alcohols

Readers

  • Immunology
  • Thermal Physics or Thermal Science.

Technology Areas

  • Space
  • Space - Hall-Effect Thruster