SEROLOGICAL SURVEY FOR HERPES ANTIBODIES AMONG A POPULATION IN A LIMITED AREA OF TOKYO AND VICINITY AND ATTEMPTS TO GROW HERPES B VIRUS AND RICKETTSIA ORIENTALIS IN THE ONE-DAY OLD EGG
Abstract
Nineteen more serum samples obtained from outpatients of a hospital and 23 serum samples taken from adult personnel were subjected to nasotracheal (NT) and complement-fixation test (CFT). The data substantiated our previous conclusion that during the first year since birth only a minor or doubtful level of antibody remained by NT but mostly negative titers were obtained by CFT, and from the age of 3 years on both the NT AND CFT antibodies showed significant rises up to the age of 5 yr, after which about 50% of population revealed the presence of antibodies. Among adults, this ratio was about 60%. In order to know what changes have occurred recently in herpes antibody distribution among the Tokyo population, a retrospective study is now going on using serum samples taken and frozen during the past two years. When a freshly isolated herpes B virus was inoculated in 1-day eggs, a luxuriant growth of virus was seen, the highest concentration being found in the CAM-yolk sac portion. The virus growth was better than that occurring after inoculation of 12-day eggs. The virus at the 2nd to 3rd egg passages showed a high 1-day-egg-cidal action so that it could be titrated by the 1-day egg LD titration. Two strains of Rickettsia orientalis, Kato and Karp, were tested for growth in 1-day eggs. Quite different from the cases of virus infection of eggs, the 1-day egg was far less susceptible to the rickettsial infection than older eggs. Serial 1-day egg passages resulted in the loss of rickettsia after 2 to 4 passages. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 20, 1961
- Accession Number
- AD0255138
Entities
People
- Kamesaburo Yoshino
Organizations
- Istituto Superiore di SanitÃ