THE CULTIVATION OF DENGUE-1 (HAWAIIAN) VIRUS IN TISSUE CULTURE. III. CYTOPATHOGENIC VIRUS SUB-CULTURED FROM HUS 2806-DENGUE-1 CARRIER CULTURE
Abstract
Dengue-1 virus has been maintained in human skin cell carrier culture for 2 years. Subsequent to continuous propagation for 15 months, a variation in the cytopathogenicity was observed, viz., the regular production of an acute cytopathic effect (CPE) associated with infection of human skin (HuS 2806) and monkey kidney (MKE) cell cultures, in contrast with the lack of any significant CPE when similar cultures were infected with the mouse-adapted virus. In cultures of either cell type a minimum of 10 to 14 hr contact time between cells and virus was necessary for maximum infectivity as measured by CPE. Using CPE as the indicator, virus titrations of infectious fluids were completed in 5 days with HuS 2806, and in 10 days with MKE cell cultures. The association of CPE with virus reproduction was shown in two ways. Using suckling mice for assay the growth curves of cell-adapted virus showed peak titers of 10 to the 4th power mouse LD50 in HuS 2806 cell cultures after 78 hr, and 10 to the 5th power mouse LD50 in MKE cell cultures after 125 hr, corresponding with the development of CPE in each case. Using both mice and tissue cultures to assay numerous harvests and pools of tissue culture fluid containing up to 10 to the 7th power infectious units, the titration endpoints for CPE (TC CPD50) were comparable to the mouse LD50 endpoints of duplicate samples. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 04, 1960
- Accession Number
- AD0256395
Entities
People
- I.e. Fishburne
- N.h. Wiebenga
Organizations
- Naval Medical Research Center