FURTHER OBSERVATIONS ON KETONE BODY AND FATTY ACID OXIDATION BY DIAPHRAGM FROM NORMAL RATS AND RATS FASTED IN A COLD ENVIRONMENT
Abstract
The minimal calculated percent of CO2 of excised rat diaphragm produced from C14-ethyl acetoacetate, C14-sodium beta hydroxybutyrate and C14-sodium palmitate was determined using tissue from normal rats and from rats starved for 24 hours in a 4 C. environmental temperature. There was no marked difference between the normal fed animal and the cold-starved animal under the conditions of these experiments. Unlabeled glucose did not augment the oxidation of labeled palmitate by diaphragm from either the normal or cold-starved animal. Acetoacetate is more extensively converted to CO2 than is beta hydroxybutyrate. When both substrates are present as a mixture, acetoacetate contributes twice as much to the CO2 as does beta hydroxybutyrate. The marked depression of C14O2 from labeled palmitate when unlabeled acetoacetate is present, is consonant with the theory that labeled palmitate mixes with the intracellular source of CO2 and that the minimal calculated percent of CO2 in the case of labeled palmitate is much lower than true value for contribution of palmitate to the CO2. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 04, 1960
- Accession Number
- AD0256398
Entities
People
- E.m. Jr. Neptune
- F.j. Fash
- W.w. Shreeve
Organizations
- Naval Medical Research Center