THE INFLUENCE OF CHOLESTEROL ON THE DETERMINATION OF SERUM GLYCERIDES

Abstract

The development of a direct method for the determination of serum glycerides has increased the accuracy of this determination. However, the influence of interfering substances has received little attention. Since a lipid extract is employed, the only interfering substances would be lipid in nature, i.e., cholesterol and fatty acids. Cholesterol has been shown to interfere with the color development in the direct procedure. A chloroform extraction has been introduced in the procedure which removes cholesterol and fatty acids, thereby preventing any interference from these compounds. (Author)

Document Details

Document Type
Technical Report
Publication Date
Feb 20, 1961
Accession Number
AD0256794

Entities

People

  • G.a. Leveille
  • H.e. Sauberlich
  • J.w. Shockley

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Biomolecules
  • Chemical Compounds
  • Chloroform
  • Cholesterol
  • Extraction
  • Fatty Acids
  • Glycerides
  • Lipids

Readers

  • Analytical Chemistry