THE INFLUENCE OF TIME ON THE DEATH OF HELA CELLS AT ELEVATED TEMPERATURES

Abstract

The time required to kill HeLa cells in suspension was determined for the range of temperatures from 48 to 65 C. The cells were placed between 6-micron films of polyethylene 50 microns apart. One side of this chamber was placed in a wellstirred water bath at the elevated temperature for the desired time and then held in a bath at 37 C for 15 minutes. Eosin-Y, mixed with the cell suspension before exposure, stained the cells affected by the elevated temperature episode and distinguished live from dead cells. The time (t) to cause death of the HeLa cells was found to range from 1 second at 65 C to 1900 seconds at 48 C. (Author)

Document Details

Document Type
Technical Report
Publication Date
Dec 19, 1960
Accession Number
AD0259671

Entities

People

  • George Jr. Mixter
  • Guy P. Delhery

Tags

DTIC Thesaurus Topics

  • Dielectric Polymers
  • Dielectrics
  • Macromolecules
  • Materials
  • Molecules

Readers

  • Military History of the United States in the 20th Century.
  • Oncology (Cancer Research).
  • Thermal Physics or Thermal Science.