THE MECHANISM OF PHOTOPHOSPHORYLATION IN CHLORO-PLASTS
Abstract
Utilizing O-18 labelled substrates, it was established that 1 atom of O enters from the water of the medium into each ATP molecule produced. In addition the location of the phosphate oxygens, the possibility of interfering side reactions and the source of the new bridging oxygen atom in the ATP molecule were determined. The inhibitory effect of UV irradiation on the Hill reaction and photophosphorylation was quantitatively determined. A positive correlation was established between the destruction of coenzyme Q sub 255 and the inactivation of the photosynthetic reactions brought about by UV irradiation. By taking advantage of the properties of a photophosphorylative system, ATP or ADP labelled at specific and different sites with P-32 was synthesized. By a modification, the method could be used for the synthesis of many other P-32 labelled compounds. Light activated Pi-ATP exchange was discovered to be present in chloroplasts. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 30, 1961
- Accession Number
- AD0262091
Entities
People
- M. Avron
Organizations
- Weizmann Institute of Science