HAPTENIC PROPERTIES OF PARALYTIC SHELLFISH POISON

Abstract

Through additional modification of the procedures for coupling diazo- PSP to proteins, a product has been synthesized of sufficiently low toxicity to be acceptable for use as an antigen and containing at least 10 times the amount of PSP as previous preparations. Immunological studies of PSP-bovine gamma II globulin, PSP-ovalbumin and their respective controls provided conflicting evidence regarding the haptenic properties of the PSP portion of the conjugates yet to be reconciled. Evidence has been presented to indicate PSP enters into a reversible equilibrium reaction with nitrous acid and that the diazotization does not appear to drastically alter the PSP molecule. In search of alternate methods for chemical assay procedures, a color reaction between PSP and sodium 1,2,-napthoquinone-4-sulfonate at pH 9.3 has been investigated in terms of specificity and sensitivity.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1961
Accession Number
AD0263132

Tags

DTIC Thesaurus Topics

  • Absorption Coefficients
  • Acids
  • Albumins
  • Antibodies
  • Assays
  • Bioassay
  • Biological Laboratories
  • Chemical Equilibrium
  • Chemical Reactions
  • Globulins
  • Government Procurement
  • Health Services
  • Immune Serums
  • Molecules
  • Nitrogen Compounds
  • Proteins
  • Public Health

Readers

  • Microbial Pathology
  • Organic Chemistry
  • Systems Analysis and Design