MISCELLANEOUS NOTES PERTAINING TO TISSUE STAINING PROCEDURES
Abstract
Three of the spot tests used for identification of molybdenum in the test tube have been adapted for histochemical use. With the benzidine and stannous chloride methods only the localization of molybdenum can be demonstrated. However with the thiocyanate method free and combined molybdenum can be differentiated. With all three methods, the moly denum must initially be in the +8 oxidation state. With the thiocynate method it was possible to demonstrate that in those structures stained by fiber stains in trichrome procedures there was an actual combination between the phosphomolybdic acid and the fiber. The relative acidophilia of various tissue elements may be assessed by controlled staining with Lendrum's phloxine tartrazine method. If the staining bath is maintained at 60 C, the staining time with Mallory's phosphotungstic acid hematoxylin may be reduced to 2-1/2-5 hours. The resulting stain is also more intense than when the original method is used. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 28, 1961
- Accession Number
- AD0264977
Entities
People
- Gene R. Berube
- George Clark
- Margaret M. Powers
Organizations
- United States Army Medical Research Laboratory