FATTY ACID METABOLISM IN RAT INTESTINAL SEGMENTS
Abstract
An in vitro prepara ion of rat intestinal segments capable of incorporating, esterifying and o idizing f tty acids is presented. After appropriate selection of tissue segments, reproducibility is excellent. With this pr paration, three separate reac io hav be n s udied; esterification, oxidation, and incorporation of fatty acid. E erification re uired glucose a d oxygen but was insensitive to insulin. Succinate did no substi u for glucose. Oxidation and esterification were depressed by succina , fluoride, and arsenate. Oxidation a not influenced by glucose. I corporation followed the ki tic of first order reaction at any albumin concentration. It was also found to be a rever ible reaction, indep ndent of cellular energy production. The implic tions of the reversible nature of the reaction have been discussed wi r ference to a reinterpretation of the source of plasma FFA. Sodium taurocholate in high concentration accelerated incorporation, but not esterification, as h s been previously reported. (Au or)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 21, 1961
- Accession Number
- AD0265261
Entities
People
- C. Entenman
- D. Porte
Organizations
- Naval Radiological Defense Laboratory