MECHANISM OF ENZYME ACTION

Abstract

The research herein described has been an investigation of the mechanism of catalysis of proteolytic enzymes in general, and of alphachymotrypsin specifically. This was undertaken both by degradative studies of alpha-chymotrypsin itself and by the synthesis and kinetic investigation of possible model systems, both polymeric and of low molecular weight. An interesting effect recently discovered was the increase of catalytic rate constant (or catalytic activity) with increasing amount of imidazole in the polymers, independent of imidazole-group concentration in the solution, and no matter what the other groups in the polymer. Explanations for this phenomenon are being sought. The synthesis of imidazole-containing monomers, polymers, and low molecular weight model compounds, and the kinetic investigation of these species as catalysts, in the search for model systems of the proteolytic enzymes and the mechanisms of their action, continue. (Author)

Document Details

Document Type
Technical Report
Publication Date
Oct 26, 1961
Accession Number
AD0265495

Entities

People

  • C.g. Overberger
  • Norman Vorchheimer
  • Thomas St. Pierre

Organizations

  • New York University Tandon School of Engineering

Tags

DTIC Thesaurus Topics

  • Catalysis
  • Catalysts
  • Chemical Reaction Properties
  • Chemical Reactions
  • Imidazoles
  • Molecular Weight

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Polymer Science and Technology
  • Theoretical Analysis.