CONDUCT AN INVESTIGATION OF CIGUATERA POISON

Abstract

Research on ciguatera toxin resulted in a satisfactory method for extracting the toxin from fish muscle. Partial purification was also accomplished by precipitation and silicic acid adsorption. The most precise fractionation of ciguatera toxin is accomplished on a silicic acid column developed in a stepwise fashion by other containing small percentages of methanol. It is felt that further experiments using silicicACID COLUMN CHROMATOGRAPHY WILL LEAD TO HIGHLY PURIFIED SAMPLES OF CIGUATERA TOXIN. Paper chromatography was examined using fourteen different solvent systems, but none proved satisfactory. A toxic fraction was found in the bluegreen alga, Lyngbya majascula from Palmyra Island which corresponds to the ciguatera fraction on the silicic acid column. This latter find may be a lead as to the source of the poison in nature, and may be of considerable CW-BW significance. (Author)

Document Details

Document Type
Technical Report
Publication Date
Nov 07, 1961
Accession Number
AD0266789

Entities

People

  • Bruce W. Halstead
  • Donald W. Hessel

Tags

DTIC Thesaurus Topics

  • Adsorption
  • Alcohols
  • Analytical Chemistry Techniques
  • Chemical Compounds
  • Chromatography
  • Column Chromatography
  • Fractionation
  • Methanols
  • Organic Compounds
  • Precipitation

Readers

  • Analytical Chemistry
  • Aquatic Ecology
  • Regression Analysis.