STUDIES ON THE NATURE OF THE METABOLIC RESPONSE TO INJURY
Abstract
Studies of adrenal and extra-adrenal actions of three modifications of ACTH involving only the N-terminal serine indicate that an intact serine is necessary for maximal in vitro action of ACTH on the adrenal cortex but not for extra-adrenal action on adipose tissue in vitro or on adrenal cortex in vitro. A free terminal alpha-amino N is necessary for the latter activities, whereas both an alpha-amino N and a beta-OH are required for in vivo action on the adrenal cortex. Using various synthetic peptides related to ACTH it was found that the minimal chain length for action of adipose tissue is the same as for action on the adrenal cortex, e.g. between 13 and 18 amino acid residues. An extra-adrenal diabetogenic effect of ACTH in cortisone maintained adrenalectomized rats was demonstrated. Factors involved in peroxidation of unsaturated fatty acids during incubation of adipose tissue in vitro have been studied in depth. The role of the pituitary and of its hormones in the concentration of alphaamino isobutyric acid C(14) and unlabeled proline and alanine by rate diaphragm has been investigated. Growth hormone has a specific effect on AIB transport, shared to only a slight degree by prolactin. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1961
- Accession Number
- AD0267601
Entities
People
- Frank L. Engel
Organizations
- Duke University