The Far Ultraviolet Absorption Spectra of Polypeptide and Protein Solutions and Their Dependence on Conformation,
Abstract
The absorption spectra of polypeptides and proteins in aquenous solution in the region 185-240 millimicron were investigated and various factors contributing to this absorption were analysed. In polypeptides in the alphahelical form a large hypochromic effect is observed as well as a new feature in the form of a shoulder at 205 millimicron, which is not present in the spectrum of the random coil conformation. The absorption spectrum associated with the beta-form was recorded, and is shown to differ from each of the other two. The hypochromic effect is also observed in proteins, and by applying a correction for the absorption of chromophoric side chains, it is possible to arrive at a value for the helical content of the protein, hich agrees moderately well with data derived from optical rotatory dispersion measurements. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1961
- Accession Number
- AD0267903
Entities
People
- K. Rosenheck
- P. Doty
Organizations
- Harvard University