INTERRELATIONSHIPS IN ENRICHMENT CULTURE OF AQUATIC AND MARINE BACTERIA
Abstract
The objectives of this research have been (1) to develop methods for the investigation in the laboratory of factors which control the rise and fall of populations of particular aquatic and marine microorganisms (2) to study effects of specific nutritive and environmental factors on the microbial flora in open systems which simulate the stream or ocean and, (3) to study the interrelationships between the organisms which develop in these steady-state enrichment cultures. Earlier it was found that when sea water was passed continuously over a bed of decomposing algae and fed into a culture chamber, prolific growth of large colorless sulfur bacteria ensued. All attempts to grow these bacteria on conventional liquid and solid media were unsuccessful. In this steady-state enrichment culture hydrogen sulfide, generated by reduction of sulfate from sea water, is continuously provided at low concentrations, thereby providing an energy source for colorless sulfur bacteria. Sphaerotilus was cultured in a set of six stainless steel vessels and the effects of certain variables noted. Good growth is obtained in a medium consisting of glucose and mineral salts, with ammonia as the nitrogen source and tap water as the diluent. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1961
- Accession Number
- AD0268008
Entities
People
- Erling J. Ordal
- F.e. Palmer
Organizations
- University of Washington