STEROID-PROTEIN INTERACTIONS. VIII. STRUCTURE OF DELTA (4)-3-KETOSTEROIDS IN RELATION TO SPECTROPHOTOMETRIC INTERACTION WITH HUMAN SERUM ALBUMIN
Abstract
Spectrophotometric observations show that introduction of methyl into delta (4)-3-ketosteroids strengthens the interaction with human serum albumin (HSA). Increase of the electron density at the rear side of the steroid molecule weakens this interaction. Simple alpha, beta-unsaturated ketones interact with HSA in a way similar to delta (4)-3-ketosteroids. The spectrometric results on the influence of functional groups in delta (4)-3-ketosteroids on interaction with HSA are summarized. Observation of bathochromic shifts in the ultraviolet absorption of 6 betamethyl-substituted delta (4)-3-ketosteroids confirms the concept of Ringold and Bowers that the inductive effect of a 6 beta-axial substituent is greater than that of the corresponding 6 alpha substituent. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 20, 1961
- Accession Number
- AD0271068
Entities
People
- Billy D. Ashley
- Ulrich Westphal
Organizations
- United States Army Medical Research Laboratory