SYNTHETIC POLYMER STUDIES RELATING TO ENZYMOLOGY
Abstract
An attempt to synthesize a polymer with a specific affinity for cholinesterase by attaching m-hydroxy trimethylanilinium functions to the polymer backbone was abandoned. The hydrolysis of m-acetoxytrimethylanilinium iodide catalyzed by singly, doubly and triply ionized citric acid showed the doubly ionized citrate to have an anomalously low catalytic activity. The hydrolysis rate of B-N,N-dimethylamino ethyl hydrogen phthalate was studied over a range of pH values. The base catalyzed hydrolysis rate of phthalamide and methyl phthalamate was very high due to the attack of the conjugate base of the amide on the amide of ester function. The hydrolysis rate of N-(o-carboxy) phenylphthalamic acid was studied over a range of pH values. The rate is a maximum when one of the 2 carboxyl groups is ionized. The hydrolysis of a compound carrying 2 cationic groups and 2 ester groups was studied in buffers and in partially ionized polymeric acids. The polyanions were found to act as powerful inhibitors for the hydroxyl ion catalyzed reaction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 28, 1962
- Accession Number
- AD0273645
Entities
People
- H. Morawetz
- J. Shafer
Organizations
- New York University Tandon School of Engineering