EFFECTS OF SUGARS ON THE SURVIVAL OF SERRATIA MARCESCENS AFTER FREEZE DRYING OR AEROSOLIZATION AT UNFAVORABLE HUMIDITY

Abstract

Suspensions of Serratia marcescens were subjected to freeze drying or to aerosolization at unfavorable humidity levels. The survival of the cells during these treatments was markedly improved in the presence of common sugars, but no one sugar stabilized the cells against both stresses. The protective effects of the sugars were correlated with their penetrability into cells: minimally penetrable (MP) sugars stabilized cells against aerosolization and freely penetrable (FP) sugars stabilized cells during freeze drying. These results were attributed to the modifications of intracellular water content induced by the presence of the sugars in the cell suspensions.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1962
Accession Number
AD0276169

Entities

People

  • Leonard Zimmerman

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anthrones
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Evaporation
  • Freeze Drying
  • Fungi
  • Humidity
  • Macromolecules
  • Materials
  • Microorganisms
  • Molecules
  • Monosaccharides
  • Organic Chemistry
  • Sugars

Fields of Study

  • Biology

Readers

  • Mathematics or Statistics
  • Microbial Pathology