CHEMICAL FACTORS IN THE EXCITATION OF CEREBRAL TISSUES
Abstract
Apparatus and techniques are described for maintaining isolated mammalian cerebral tissues in an excitable condition. Their excitability has been demonstrated by three types of observation: (1) Measurement of change in chemical composition in response to electrical stimulation. Most attention has been paid to changes in K, Na, and phosphocreatine; (2) Resting membrane potentials have been measured by glass micropipette electrodes, and depolarization demonstrated after applying brief electrical pulses to the tissue; and (3) Occasional spike discharges have been observed on penetrating the tissue with micropipette electrodes. Association between chemical and electrical events in the isolated tissue has been demonstrated in the following situations: (1) During and after electrical stimulation the time-course of change in potential and in Na and K content are similar; and (2) The basic protein clupein was effective in inhibiting active ion movements and in preventing the establishment of normal resting potentials, and in both these actions it was antagonized by ganglioside preparation. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- May 14, 1962
- Accession Number
- AD0278673
Entities
People
- Henry Mcilwain