HOMOLOGOUS BONE MARROW TRANSPLANTATION IN DOGS RECEIVING X RADIATION PLUS URETHANE OR 6-MERCAPTOPURINE
Abstract
Experience has now been accumulated with homologous marrow transplantation in 13 mongrel dogs (Group 1) treated with 6-mercaptopurine (6-MP) or urethane prior to X irradiation (900 r, delivered at a dose rate of 15 r/minute). The marrow dose was 7 to 11 billion cells, given on the day following irradiation; urethane (175 or 350 mg/kg) and 6-MP (12.5 or 25 mg/kg) were administered at 3 or 4 daily intervals during the week prior to irradiation. Mean survival time (MST) in Group 1 was 23 days, with a maximum of 63 days. MST in a group of dogs (Group 2) given homologous marrow after 900 r, but not treated with the chemicals, was 10 days. The marrow showed active hematopoiesis and moderate to good cellularity in most of the Group 1 animals, although megakaryocyte activity was deficient in some. Pneumonia and pulmonary edema were found in many of the dogs, at autopsy. The use of these anti-metabolites permits the successful transplantation of homologous marrow in dogs at a dose of X radiation (900 r) which, by itself, is insufficient. Urethane and 6-mercaptopurine are additive to X radiation with respect to suppressing the homograft reaction in dogs, as well as in mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 19, 1962
- Accession Number
- AD0282035
Entities
People
- E. L. Alpen
- Leonard J. Cole
Organizations
- Naval Radiological Defense Laboratory