WOUND HEALING, COLLAGEN, AND HUMORAL GROWTH-PROMOTING AGENTS
Abstract
Physical, biochemical, surgical and histological techniques were used in a series of studies on many aspects of wound healing. Acetic acid solubilized collagen is polydisperse and this mixture was fractionated into 3 high molecular weight components and a peptide complex. While the three high molecular weight components resemble the parent collagen molecule very closely in their amino acid composition, they do differ slightly but significantly from one another. The peptide fraction was separated into some 14 fractions; they have little hydroxyproline and much proline; they may represent an early stage in collagen development, that is, they are precursors. Evidence to substantiate this hypothesis was obtained through metabolic studies using C14 glycine. Dog wound fluid ultrafiltrate, a low molecular weight component which is a growth accelerator for bacterial and mammalian cells, was isolated. An in-vivo collagen defect was created in rats by feeding them deuterium oxide. This technique provided a tool for the study of the interaction of collagen with the ground substance; this method was applied to elucidate how the mucopolysaccharides of ground substance stabilize collagen, a vital subject about which little is now known.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1962
- Accession Number
- AD0286050
Entities
People
- C. W. Berard
- E. F. Greever
- H. Rosen
- S. M. Levenson
Organizations
- Walter Reed Army Institute of Research