DETECTION AND MEASUREMENT OF ANTIGEN-ANTIBODY REACTIONS
Abstract
The following aspects of a program to develop methods for the detection and measurement of antigen - antibody reaction using the fluorescent labelling technique are reported: (1) a technique was developed for growing turnip yellow mosaic virus and producing antibody in rabbits, providing a suitable immunological system for the current investigation, (2) successful experiments were performed using abelled diptheria toxoid as a reagent to detect diptheria antibodies, (3) disintegration of bacteria to form fragments of low molecular weight suitable for the technique under consideration was achieved and antibodies to the cell walls prepared, and (5) instrumentation sensitivity an reliability were improved by changes in the optical and detecting systems, as well as by the incorporation of additional controls.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 1962
- Accession Number
- AD0286321
Entities
People
- William P. Murphy Jr.