RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD. XI. DEVELOPMENT AND CLINICAL EVALUATION OF PROCESSES FOR THE LOW TEMPERATURE PRESERVATION OF BLOOD BASED ON RAPID COOLING AND WARMING AND PROTECTION BY POLYMERS
Abstract
Four basic processes for low temperature blood preservation were formulat d n evaluated at a laboratory level and in animal and human transfusions. Process I involves separating the red cells from blood by conventional ce trifugation, resuspending the red cells and freezing in a polymer-containing medium, and after thawing resuspending the cells in their plasma for transfusion. Polyvinylpyrrolidone (PVP) was studied extensively in this process. Process II involves freezing the red cell fraction of blood in the presence of part or all of the plasma combined with polymeric additives. Process III involves freezing whole blood collected into or combined after collection with polyvinylpyrrolidone at a concentration of about 7% w/v. At this concentration of PVP quantities of frozen blood up to 400 ml were transfused to human recipients without post-thaw processing of any kind. No a v rse clinical effects were observed. Process IV involves freezing the r d cell fraction of blood in the presence of a small volume (about 25% of the cell volume) of polyvinylpyrrolidone-plasma or human serum albumin. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1962
- Accession Number
- AD0286754
Entities
People
- A. P. Rinfret