CHROMOSOMAL STUDIES ON IRRADIATED LEUKOCYTES IN VITRO
Abstract
The technic for cultivation of the human buffy coat with heparinized venous blood treated with phytohemagglutinin was described. By this method chromosome spreads were made from 6-day cultures, using the colchicine-hypotonic, sodium citrateacetic, orcein-squash sequence. The range of polyploidy was determined on 2 specimens from each of 3 individuals (2 males and 1 female). Detailed analyses and counts of human chromosomes indicated the predominant distribution to be euploid cells having 46 chromosomes. Idiogram studies showed the same basic pattern demonstrated by previous investigators. These data served as a basis for comparing results obtained from sister cultures irradiated with 400 r from a cobalt-60 source on the fourth day and fixed on the sixth day. There was a considerable increase, 6.3 percent, in the number of polyploid cells. Among these, tetraploid cells were the most frequent, being 5.4 percent. By chromosomal analyses, 56.6 percent of the cells were found to be damaged. Most injuries were due to chromosomal breakages, especially fragments and dicentrics. Endoreduplication was noted frequently. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1961
- Accession Number
- AD0287067
Entities
People
- C.m. Pomerat
- Y. Ohnuki
Organizations
- United States Air Force School of Aerospace Medicine