THE GROWTH OF ASIBI STRAIN YELLOW FEVER VIRUS IN TISSUE CULTURES. I. SENSITIVITY AND CAPACITY OF TISSUE CULTURES

Abstract

A number of primary and established tissue cultures were examined for their susceptibility to yellow fever virus, with the most intensive study directed to HeLa cells. The following results were obtained: Of those cultures that were susceptible, three different patterns of dose response were obtained that were interpreted as different possible manifestations of interference. HeLa cells, which were among the least sensitive to low infection multiplication, had the greatest capacity (highest yields). Subjecting cultures to wash cycles between 15 minutes and 2 hours post-inoculation increased the sensitivity (detectable virus) of such cultures 100-fold, increased the rate of virus multiplication, and had no effect on the peak titer (capacity). The HeLa cultures were found to be composed of different (morphological) clonal types. Three of these that were studied were all susceptible, comparable in capacity, but varied in their sensitivity.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1962
Accession Number
AD0288865

Entities

People

  • Frank M. Hardy

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Arboviruses
  • Biological Laboratories
  • Cell Line
  • Cells
  • Culture Techniques
  • Epithelial Cells
  • Infection
  • Inoculation
  • Rodents
  • Sensitivity
  • Tissue Culture
  • Virus Diseases
  • Viruses
  • Wound Infections
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).