THE GROWTH OF ASIBI STRAIN YELLOW FEVER VIRUS IN TISSUE CULTURES. II. MODIFICATION OF VIRUS AND CELLS

Abstract

Passage of the parent Asibi strain yellow fever virus in HeLa cell culture through six consecutive passages produced a variant (H-6) that, unlike P virus: (a) induced cytopathic effect in HeLa and chick fibroblast cell cultures, (b) became more virulent for mice, and (c) lost its ''viscerotropic'' character for the monkey. Chronic infection of HeLa cells with P virus was established for a two-month period. The virus obtained from this culture showed characteristics of the P and H-6 and was designated parent chronic (Pc). The Pc virus strain was undetectable from supernatant fluid two months postinfection. Cloned populations of the surviving cells showed evidence of the modification induced by the Pc virus in terms of susceptibility (sensitivity and capacity).

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1962
Accession Number
AD0288869

Entities

People

  • Frank M. Hardy

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Biological Laboratories
  • Cell Line
  • Cells
  • Culture Techniques
  • Fibroblasts
  • Government Procurement
  • Governments
  • Immune Serums
  • Infection
  • Inoculation
  • Lethal Dosage
  • Rodents
  • Tissue Culture
  • Viruses
  • Wound Infections
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Immunology
  • Infectious Disease/Epidemiology
  • Virology (or Medical Virology).