STUDIES ON VIRAL ONCOLYSIS BY MEANS OF FLUORESCENT ANTIBODY TECHNIQUE
Abstract
Fifteen different types of dye compounds with various kinds of conjugating radicals were evaluated as to the adequacy for the fluorescent antibody technique. Three practical fluorescent antibodies were prepared, using DNS, XRB, and a simple fluorescent dye from cyanulyl chloride. DNS fluorescent antibody has a yellow fluorescence, XRB a tannish-red, and cyanulyl chloride dye a yellowish-green bright fluorescence. Each of them can easily conjugate with protein and causes no denaturation of protein during the reaction. The characteristics of these labeled proteins were investigated with Beckman's spectrophotometer and spectrofluorescentphotometer. Labeling the dyes and the latest technique of the fluorescent antibody preparation were discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 18, 1962
- Accession Number
- AD0291636
Entities
People
- Michio Ito
Organizations
- United States Army Biological Warfare Laboratories