CHARACTERISTICS OF THE POLYSACCHARIDE-CONTAINING FRACTION FROM PASTEURELLA PESTIS

Abstract

The preparation isolated by the White method from Past. pestis does not represent a pure polysaccharide. Non-purified fractions contained a considerable amount of protein admixtures, from which it could be separated via repeated, fractionated precipitation with alcohol, processing with chloroform as per Sevag, prolonged hydrolysis with 0.1 N acetic acid, or protein precipitation with trichloracetic acid. The preparation purified of protein admixtures represents a stable polysaccharide-polypeptide complex possessing serological activity and the property of inducing a positive allergic reaction in guinea pigs which had recovered from plague or had been immunized against this infection. Removal of the polypeptide portion of the complex by means of trypsin-processing led to a marked reduction of the allergic effect of the preparation.

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Document Details

Document Type
Technical Report
Publication Date
Sep 27, 1962
Accession Number
AD0291637

Entities

People

  • L. P. Pavlova
  • V. D. Yegorova
  • Ye. E. Bakhrakh

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Amino Acids
  • Biological Laboratories
  • Cells
  • Chemical Analysis
  • Government Procurement
  • Hydrolysis
  • Infection
  • Materials
  • Microbiology
  • Microorganisms
  • Nitrogen
  • Polysaccharides
  • Precipitation
  • Rodents
  • Scientific Research
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology