THERAPEUTIC PROPERTIES OF BLOOD PLASMA IN THE THERAPY OF TOURNIQUET SHOCK
Abstract
A convenient method for the sterile preparation of plasma (or other body fluids from small animals using a millipore filter system is described. Aged rat plasma is no more effective in the therapy of rat tourniquet trauma than freshly collected rat plasma. Frozen rat plasma seems to be also as effective as freshly collected plasma. None of the rat plasma preparations are as effective as aged or lyophilized human plasma. Recently collected single donor or pooled human plasma allows no survival when infused after the standard rat tourniquet trauma. Shelf-stored, pooled human plasma (6 months at room temperature) is as effective as reconstituted lyophilized human plasma (highly effective) in the therapy of this trauma. At the time significant protection is first achieved, electrophoretic studies show the appearance of a band not present in the fresh material. This band is also present in the lyophilized reconstituted plasma. Reconstituted human plasma is significantly more effective in the therapy of rat tourniquet trauma than human albumin when both are adjusted to the same total protein concentration. This observation questions whether the resuscitative effect of the human plasma is due to its oncotic effect only or to some property of the plasma present in the non albumin fraction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 26, 1962
- Accession Number
- AD0291949
Entities
People
- Morton D. Pareira