ON THE DEFENCE MECHANISMS AGAINST INFECTION
Abstract
Further purification studies were made of leucozyme C, A alpha and A beta of guinea pig leucocytes and the studies of their natures were continued. The complete separation of leucozyme from lysozyme was achieved and this preparation had no effect on the mucopeptide. Leucozyme A beta stops oxygen uptake and causes cytoplasmic destruction of nonpathogenic staphylococci. Of rabbit leucocytes, the acetic acid extract wasACTIVE ONLY FOR AVIRULENT, BUT THE HCl extract was active for avirulent and virulent pneumococci. Of guinea pig macrophages, the acetic acid extract of lyophilized cells was active for E. coli. Of rabbit macrophages, the neutral extract was active for E. coli and and avirulent strains of pneumococci. Antistaphylococcal substance found in guinea pig and bovine spleens and livers was differentiated from normal staphylococcal agglutinins. It is only bacteriostatic for actively growing cells. The role of gamma-glutamylase in passive protection of mice was examined administering with hyperimmune therapeutic sera (horse) and no protection was demonstrated. The attack point of plakin on the chain of respiratory system of B. megaterium was clarified. The electron transfer from flavoproteins to the cytochrome system was blocked. Phospholipids of cytoplasmic membrane were the main attack point of plakin and it was the cause of the damage of the electron transferring system. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1962
- Accession Number
- AD0292263
Entities
People
- Tsunehisa Amano
Organizations
- Osaka University