EXTRACTION APPARATUS FOR DETERMINATION OF TOTAL SERUM LIPIDS

Abstract

Two designs of glassware useful in measurement of total lipids in serum are described. One permits expulsion of the upper phase but increases requirements for solvent volumes. The other, a modified Erlenmeyer flask, facilitates withdrawal of the lower phase. Chilling the flask for 5 to 10 minutes in a refrigerator or deep freeze shortens the time required for separation of the two phases. Analyses of seven samples by each of three methods in triplicate showed that the modified flask gives results that average 3% higher than results by another method, but precision of both methods is the same. By using only 0.5 ml. of serum, the standard deviation of the results by either method is 2%.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1962
Accession Number
AD0295590

Entities

People

  • Carrie B. Haven
  • Dale A. Clark

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Space

DTIC Thesaurus Topics

  • Aerospace Medicine
  • Alcohols
  • Blood
  • Chloroform
  • Cooling
  • Extraction
  • Government Procurement
  • Governments
  • Materials
  • Measurement
  • Methanols
  • Microvessels
  • Phase
  • Separators
  • Specifications
  • Standards
  • Statistical Analysis

Readers

  • Analytical Chemistry
  • Thermal Physics or Thermal Science.